Ĭontrol measures are based on strict hygiene and vaccination programs. Animal brucellosis poses a barrier to trade of animals and animal products between countries and causes considerable economic losses due to abortion and fertility problems to the sheep and goat industry. It is endemic in sheep and goats in most countries of the Mediterranean basin, the Middle East, Central Asia, with only North America, North Europe, South-East Asia, and Oceania being spared. Brucellosis is present throughout the five continents and it is still an uncontrolled serious public health problem in many developing countries. melitensis has 3 biovars (1–3), highly pathogenic for humans. melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. Introductionīrucellosis, especially caused by B. 0 5) risk factors positively associated with the occurrence of brucellosis in sheep and goat flocks. 9 2, CI: 1.1–7.7) and grazing at common pasture ( O R = 2. Vaccination with Rev-1 in the last year ( O R = 2. For differentiation between vaccine strain and field strain infection, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using PstI endonuclease enzyme was used. melitensis was 27.1% (51/188) among aborted animals. These positive samples were obtained from 28 sheep and 33 goats. melitensis insertion sequence 711 ( IS711) primers on the 107 omp2 positive samples, only 61 confirmed to be positive for B. One hundred and seven (41.9%) biological samples were positive for the omp2 primers that were able to identify all Brucella species in the collected samples which were obtained from 86 aborted animals ( 8 6 / 1 8 8 = 4 5. ![]() Sampled animals belonged to 93 sheep and goat flocks that had abortion cases in the region. Two hundred and fifty five biological samples were collected from 188 animals (81 sheep and 107 goats) during the lambing season from September 2009 to April 2010 from the Mafraq region of Jordan.
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